If you’ve ever had to troubleshoot an HPLC method, you already know
there are a TON of factors that impact the quality of a separation. To
confuse things further, we chromatographers love our jargon a lot more
than we love being consistent. Understan...
HPLC method development is a bit like trying to solve a maze. The goal –
the cheese, if you will – is to develop a method that provides adequate
resolution, sensitivity, robustness and throughput. Sounds simple
enough, right? But when you begin to co...