Classical normal phase (NP) and reversed phase (RP) chromatography
should never be run on the same HPLC system without system modifications
in between. That is an old, golden rule not every LC operator knows
about anymore. With high effort, the HPLC ...
Pressure fluctuations or detector baseline drift may be reoccurring
problems faced by an LC operator. In such a case, the first inclination
may be to blame the LC system, mainly pump and detector, because they
seem to be responsible for a reliable sy...