In this webinar, Dr. John Rogers discusses an optimized proteomics sample preparation workflow for isobaric tags and highlights new tools for fractionation, sample throughput, accurate quantitation that enable researchers to answer complex biological questions. In particular, new high pH reversed phase spin columns which reproducibly and effectively fractionate peptide samples, resulting in a >100% increase in the number of unique peptides and >50% increase in unique protein identifications, as well as multiplexing strategies with TMT10plex to improve sample throughput and reduce overall analysis time will be highlighted.