Today, by utilizing isobaric labels (e.g., Tandem Mass Tags), >8,000 proteins can be routinely quantified across 10 experimental samples in 1.5 days of analysis. In this webinar both sample preparation and instrument methods used to collect large scale quantitative proteomic data will be presented by Christopher Rose. The MS3 instrument method (i.e., synchronous precursor selection MS3, SPS-MS3) that removes precursor interference and alleviates compression of measured ratios will also be highlighted.