In this webinar we present LC-MS workflows for cost-effective clinical research and forensic laboratory operation. The workflows, enabled by simple sample preparation coupled with rapid analysis on a 2-channel multiplexing LC system, include quantitation of vitamin D metabolites (25-OH-Vitamin D2 & D3) in blood serum and methylmalonic acid (MMA) in blood serum for research use, buprenorphine and its metabolite norbuprenorphine in urine, and ethanol metabolites ethyl glucuronide and ethyl sulfate (EtG/EtS) in urine for forensic use. High-throughput data quality and ease of operation for reduced labor costs and consumables cost will be discussed.