In liquid chromatography, good resolution of peaks is the goal in order to gain meaningful information.
In the market today, C18 is still the go-to chemistry for most applications, and there are lots of Reversed-phase, column choices, but when separating both charged and neutral analytes C18 alone won’t be enough to achieve the needed resolution.
This is where the unique surface chemistries of mixed-mode columns will help you.
Mixed-mode chromatography combines reversed-phase and ion-exchange in order to simplify and improve the chromatographic separation of these mixtures. Mixed-mode columns provide unique surface chemistries allowing selectivity to be controlled, and in doing so, achieve maximum resolution - often with compounds not possible by other conventional HPLC columns.
Focuses (and key learning objectives)
- Discover how mixed-mode chromatography columns work
- Determine how to easily optimize selectivity and resolution with mixed-mode chromatography
- Which types of sample work well on mixed-mode chromatography and how to match the analytes to the chromatography
- Detection systems that work well with mixed-mode chromatography methods
Who should attend
- Any chromatographers who are trying to separate multiple compounds, or complex mixtures, may have difficulties resolving their compounds of interest will want to learn about the power of mixed-mode chromatography.
- Pharmaceutical analytical laboratories - even generic impurity identification and quantification can be challenging with only a reversed-phase column.
- In food laboratories - challenging mixtures of neutral and ionic compounds can be overcome using mixed-mode chromatography.
- In Biopharmaceutical laboratories - trying to separate heterogeneous charged glycans.
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