Mixed-mode chromatography – Practical implementation

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Mixed-mode chromatography – Practical implementation

Team TFS
Team TFS

In liquid chromatography, good resolution of peaks is the goal in order to gain meaningful information.


In the market today, C18 is still the go-to chemistry for most applications, and there are lots of Reversed-phase, column choices, but when separating both charged and neutral analytes C18 alone won’t be enough to achieve the needed resolution.

This is where the unique surface chemistries of mixed-mode columns will help you.

Mixed-mode chromatography combines reversed-phase and ion-exchange in order to simplify and improve the chromatographic separation of these mixtures. Mixed-mode columns provide unique surface chemistries allowing selectivity to be controlled, and in doing so, achieve maximum resolution - often with compounds not possible by other conventional HPLC columns.


Focuses (and key learning objectives)

  • Discover how mixed-mode chromatography columns work
  • Determine how to easily optimize selectivity and resolution with mixed-mode chromatography
  • Which types of sample work well on mixed-mode chromatography and how to match the analytes to the chromatography
  • Detection systems that work well with mixed-mode chromatography methods

Who should attend

  • Any chromatographers who are trying to separate multiple compounds, or complex mixtures, may have difficulties resolving their compounds of interest will want to learn about the power of mixed-mode chromatography.
  • Pharmaceutical analytical laboratories - even generic impurity identification and quantification can be challenging with only a reversed-phase column.
  • In food laboratories - challenging mixtures of neutral and ionic compounds can be overcome using mixed-mode chromatography.
  • In Biopharmaceutical laboratories - trying to separate heterogeneous charged glycans.

Register here 

Version history
Last update:
‎07-27-2022 06:17 AM
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