Charge variant analysis of therapeutic proteins and monoclonal antibodies is a required technique for critical quality analysis. As new therapeutics are designed, labs may find themselves minimally adapting existing methods for their new product, without true consideration to how each parameter relates to method reproducibility and protein resolution. In this webinar, we will go through the various parameters one should consider when designing or updating a charge variant profile method “from the ground up” to yield the most reproducible, highly resolving profile charge variant profile.
Topics will include:
- Discussing the difference between strong and weak cation exchange columns
- Understanding differences between salt and pH gradients
- Providing method development tips for optimizing your gradient and finding the best buffers
- Development of volatile buffers for connecting cation exchange to MS
This webinar will be of benefit to anyone who would like to develop a more reliable LC-UV or LC-MS charge variant method, including researchers who have been running charge variant analysis for years, as well as new researchers in the field.