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Knowledge Base Articles

Extending Capabilities of Real-Time Database Search on the Orbitrap Eclipse Tribrid Mass Spectrometer for Multiplexed Proteomics

Yang Liu, William D. Barshop, Amirmansoor Hakimi, Jesse D. Canterbury, Romain Huguet, Rosa Viner International Hupo 2020To evaluate recent improvements to Real-Time Search for TMT SPS MS3 quantitation workflow including: 1) Close-out to prevent acqui...

Sensitive and High-throughput Single-cell Proteomics Workflow on New Quadrupole-ion trap-Orbitrap Mass Spectrometer with FAIMS Separation

Khatereh Motamedchaboki1; Maowei Dou1; Yongzheng Cong2; Romain Huguet1; Aaron M Robitaille1; David M Horn1; Yufeng Shen3; Daniel Lopez-Ferrer1; Ryan T Kelly2, 4; Ying Zhu4.ASMS 2020Traditionally, proteomics experiments are applied to large population...

High-throughput single cell proteomics analysis with nanodroplet sample processing, multiplex TMT labeling, and ultra-sensitive LC-MS

Khatereh Motamedchaboki1 , Maowei Dou1 , Yongzheng Cong2 , Romain Huguet1 , Aaron M Robitaille1 , Yufeng Shen3 , Geremy Clair4 ,Charles Ansong4 , Daniel Lopez-Ferrer1 , Ryan T. Kelly2,4, and Ying Zhu4APPLICATION NOTE 65714Understanding heterogeneity ...

Smart TMT with API

Qing YuASMS 2019 Orbitrap Power User MeetingA strategy to combine sample multiplexing with targeted proteomics assays for high throughput protein signature characterizationHarvard Medical School

High-Throughput Single Cell Proteomics Analysis with Nanodroplet Sample Processing, Multiplex TMT labeling, and Ultra-Sensitive LC-MS

Khatereh Motamedchaboki1, Maowei Dou1, Yongzheng Cong2, Romain Huguet1, Aaron M Robitaille1, Yufeng Shen3, Geremy Clair,4 Charles Ansong,4 Daniel Lopez-Ferrer1, Ryan T. Kelly2,4, and Ying Zhu4 Hupo 2019Understanding heterogeneity at single cell level...

A Modified Orbitrap Tribrid Mass Spectrometer with Real Time Search and Advanced Spectral Processing Enhances Multiplexed Proteome Coverage and Quantification Accuracy

Aaron M. Robitaille 1, Romain Huguet 1, Derek J. Bailey 1, Graeme McAlister 1, Arne Kreutzmann 2, Daniel Mourad 2, Daniel Lopez-Ferrer 1, Andreas Huhmer 1, Vlad Zabrouskov 1 Isobaric tagging strategies using Thermo Scientific™ Tandem Mass Tags™ (TMT™...

Improved Identification, Quantification Accuracy, and Workflow Efficiency using a Modified Quadrupole Orbitrap Mass Spectrometer and Tandem Mass Tags (TMT) Approach

Aaron M. Robitaille 1, Tabiwang N. Arrey 2, Markus Kellmann 2, Arne Kreutzmann 2, Daniel Mourad 2, Daniel Lopez Ferrer 1, Andreas Huhmer 1, Alexander Harder 2Multiplexed quantitation strategies using Thermo Scientific™ Tandem Mass Tags™ (TMT™) enable...

A Strategy to Combine Sample Multiplexing with Targeted Proteomics Assays for High-Throughput Protein Signature Characterization

Brian K. Erickson, Christopher M. Rose, Craig R. Braun, Alison R. Erickson, Jeffrey Knott, Graeme C. McAlister, Martin Wühr, Joao A. Paulo, Robert A. Everley, Steven P. GygiMol Cell. 2017 Jan 19;65(2):361-370. doi: 10.1016/j.molcel.2016.12.005. Epub ...

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