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Knowledge Base Articles

Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction

Dounia Djeghloul, Bhavik Patel, Holger Kramer, Andrew Dimond, Chad Whilding, Karen Brown, Anne-Céline Kohler, Amelie Feytout, Nicolas Veland, James Elliott, Tanmay A. M. Bharat, Abul K. Tarafder, Jan Löwe, Bee L. Ng, Ya Guo, Jacky Guy, Miles K. Husey...

Sampling the human population – ultra-highthroughput plasma protein profiling (uHTPPP) sample preparation for translational proteomics

Sarah Trusiak,1 Sergei Snovida,2 Bhavin Patel,2 Ryan Bomgarden,2 Emily I. Chen,1Present a robust, precise, and reproducible highthroughput sample preparation workflow for mass spectrometry-based large population cohort studies.1. Thermo Fisher Scient...

Standardized and scalable plasma protein quantitative profiling workflow using human blood based on a Thermo Scientific Q exactive platform

Jing Wang1; Sarah Trusiak1; Ryan D. Bomgarden2; Sergei Snovida2; Michelle Dubuke1, Emily I. Chen1APLICATION NOTE No. 73194To develop a high throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a s...

Standardized and scalable plasma protein quantitative profiling workflow using human blood based on a Thermo Scientific Q exactive platform

Jing Wang1; Sarah Trusiak1; Ryan D. Bomgarden2; Sergei Snovida2; Michelle Dubuke1, Emily I. Chen1APLICATION NOTE No. 73194To develop a high throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a s...

Powerful, standardized and versatile low-flow LCMS platform for deep dive proteomics and high-throughput analysis with maximum MS utilization

Oleksandr Boychenko1, Christopher Pynn1, Mike Baynham2, Jenny Ho3, Tabiwang Arrey4, and Peter Jehle1Hupo 2019Purpose: The availability and ease of sampling of blood and its constituents make it ideal for large cohort clinical research studies. Protei...

Standardized and scalable plasma protein quantitative profiling workflow using human blood based on a Thermo Scientific Q exactive platform

Jing Wang1; Sarah Trusiak1; Ryan D. Bomgarden2; Sergei Snovida2; Michelle Dubuke1, Emily I. Chen1APLICATION NOTE No. 73194To develop a high throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a s...

Antibody subunit analysis workflow on a quadrupole-Orbitrap mass spectrometer: from optimized sample preparation to data analysis

1. Kai Scheffler 2. Eugen DamocAPPLICATION NOTE 72854The goal of this application note is to discuss the importance of proper sample preparation for mAb subunit analysis and to provide optimized LC/MS conditions to achieve conclusive information on t...

Optimized DDA+ and HR-DIA workflows for standardized, reproducible, precise and robust label-free quantitation of proteomes

Aaron S. Gajadhar1, Oleksandr Boychenko2, Xin Zhang3, Yue Xuan4, and Andreas HuhmerASMS 2018Purpose: To overcome the limitations of current label-free proteome quantitation (LFQ) protocols, we sought to develop an end-to-end, complete LFQ workflow so...

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