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Knowledge Base Articles

Multi-pathway signaling analysis using a synthetic phosphopeptide panel, standardized sample preparation kits and SureQuant internal standard targeted quantitation

Aaron S. Gajadhar1, Bhavin Patel2, Penny Jensen2, Sebastien Gallien3,4, Romain Huguet1, Kay Opperman2, John C Rogers2, Andreas Huhmer1, Daniel Lopez-Ferrer1 International Hupo 2020To develop a universal phosphopeptide enrichment and internal standard...

Extending Capabilities of Real-Time Database Search on the Orbitrap Eclipse Tribrid Mass Spectrometer for Multiplexed Proteomics

Yang Liu, William D. Barshop, Amirmansoor Hakimi, Jesse D. Canterbury, Romain Huguet, Rosa Viner International Hupo 2020To evaluate recent improvements to Real-Time Search for TMT SPS MS3 quantitation workflow including: 1) Close-out to prevent acqui...

An Ultra High-throughput Plasma Protein Profiling (uHTPPP) Workflow Using a Modified Quadrupole-Orbitrap Mass Spectrometer

Michelle L. Dubuke1, Sarah Trusiak1, Sitara Chauhan1,Ryan D. Bomgarden2, Sergei Snovida2, Bhavin Patel2, Emily I. Chen1.An automated plasma protein profiling workflow on the new Orbitrap Exploris 240 mass spectrometer can deliver the consistency, sen...

Detecting Stable Isotope Labeled Metabolites in Untargeted Analysis Using a Benchtop Orbitrap Mass Spectrometer and a Single Processing Software Platform

Amanda Souza, Ioanna Ntai, Tatjana Talamantes and Ralf TautenhahnPurpose: Develop an integrated mass spectrometry-based workflow for stable isotope labeling (SIL) in untargeted metabolomics covering data acquisition and analysis in a single experimen...

Sensitive and High-throughput Single-cell Proteomics Workflow on New Quadrupole-ion trap-Orbitrap Mass Spectrometer with FAIMS Separation

Khatereh Motamedchaboki1; Maowei Dou1; Yongzheng Cong2; Romain Huguet1; Aaron M Robitaille1; David M Horn1; Yufeng Shen3; Daniel Lopez-Ferrer1; Ryan T Kelly2, 4; Ying Zhu4.ASMS 2020Traditionally, proteomics experiments are applied to large population...

Turnkey, Multi-pathway Signaling Analysis Using a Synthetic Phosphopeptide Panel, Standardized Sample Preparation Kits and SureQuant Internal Standard Targeted Quantitation

Aaron S. Gajadhar1; Bhavin Patel2; Penny Jensen2; Sebastien Gallien3,4; Romain Huguet1; Kay Opperman2; John C Rogers2; Andreas Huhmer1; Daniel Lopez-Ferrer1 ASMS 2020Purpose: We sought to develop a universal phosphopeptide enrichment and internal sta...

Pushing the Limits of Sensitivity: Micropillar Array-Based Chromatography Coupled to a Quadrupole Orbitrap Mass Spectrometer and FAIMS for Low-Input Proteomics

Julia Kraegenbring1, Karel Stejskal2, Claudia Ctortecka2, Gabriela Krššáková2, Jeff Op De Beeck3, Tabiwang N. Arrey1, Bernard Delanghe1, Alexander Harder1, Karl Mechtler4ASMS 20201 Thermo Fisher Scientific, Hanna-Kunath-Str. 11, Bremen, Germany. 2 In...

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