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Knowledge Base Articles

Multi-pathway signaling analysis using a synthetic phosphopeptide panel, standardized sample preparation kits and SureQuant internal standard targeted quantitation

Aaron S. Gajadhar1, Bhavin Patel2, Penny Jensen2, Sebastien Gallien3,4, Romain Huguet1, Kay Opperman2, John C Rogers2, Andreas Huhmer1, Daniel Lopez-Ferrer1 International Hupo 2020To develop a universal phosphopeptide enrichment and internal standard...

Turnkey, Multi-pathway Signaling Analysis Using a Synthetic Phosphopeptide Panel, Standardized Sample Preparation Kits and SureQuant Internal Standard Targeted Quantitation

Aaron S. Gajadhar1; Bhavin Patel2; Penny Jensen2; Sebastien Gallien3,4; Romain Huguet1; Kay Opperman2; John C Rogers2; Andreas Huhmer1; Daniel Lopez-Ferrer1 ASMS 2020Purpose: We sought to develop a universal phosphopeptide enrichment and internal sta...

Real-time, High Density Monitoring of pTyr Signaling Targets in Human Tumors Using Heavy Peptide Triggered Targeted Quantitation

Aaron S. Gajadhar1, Lauren E. Stopfer2, Cameron T. Flower2, Forest M. White2, Bhavin Patel3, Sebastien Gallien4, Romain Huguet1, Graeme McAlister1, Derek Bailey1, Shannon Eliuk1, Markus Kellmann5, Tabiwang N. Arrey5, Alexander Harder5, Daniel Lopez-F...

Quantitative, comprehensive multi-pathway signaling analysis using an optimized phosphopeptide enrichment method combined with an internal standard triggered targeted MS assay

Bhavin Patel1; Penny Jensen1; Aaron S. Gajadhar2; Sebastien Gallien3; Jae Choi1; Romain Huguet2; Graeme McAlister2; Derek Bailey2; Shannon Eliuk2; Markus Kellmann4; Tabiwang N. Arrey4; Alexander Harder4; Andreas Huhmer2; Kay Opperman1; John C Rogers1...

SMOAC (Sequential Enrichment from MOAC, “Smoke”), a phosphoproteomics strategy for the separation of multiply phosphorylated from monophosphorylated peptides

Jae Choi, Sergei I. Snovida, Ryan Bomgarden, John C. RogersASMS 2017 PosterPurpose: We explored sequential methods for complementary phosphopeptide enrichment to find an effective strategy for enrichment of multiply phosphorylated peptides. We also e...

Quantitative Analysis of Two Cancer Signaling Pathways Using Multiplex Immunoprecipitation and Targeted Mass Spectrometry

Bhavin Patel1, Penny Jensen1, Abid Haseeb1, Leigh Foster1, Gregory Potts2, Kay Opperman1, Rosa I Viner3, Andreas Huhmer3, John C Rogers1Introduction: The objective of this study was to determine the efficacy of multiplex IP to targeted MS (mIP-tMS) t...

Pushing the leading edge in protein analysis: Thermo Scientific Q Exactive HF-X hybrid quadrupole-Orbitrap mass spectrometer

BrochureUnderstanding biomolecules as drug targets, disease markers, and therapeutic agents demands highly sensitive analysis of complex biological samples, without sacrificing robustness and speed. The Thermo Scientific™ Q Exactive™ HF-X hybrid quad...

Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer - The Most Powerful Instrument Designed to Advance Your Scientific Pursuits

Orbitrap Fusion Lumos MS BrochurePUSHING NEW FRONTIERS IN SCIENCE Breakthroughs in science rely on great scientists making rapid progress answering challenging questions. “How does lipid metabolism affect cancer?” “How do non-cancerous cells mutate?”...

A Journey in Biotherapeutic Peptide Mapping

Cheng G C. https://www.youtube.com/watch?v=boL6vZ4s4hkLearn about how the latest chromatography, mass spectrometry and software are accelerating confident decision making at Alexion Pharmaceuticals, Inc. Discover how this biopharmaceutical company, f...

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