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Knowledge Base Articles

A robust mass spectrometer for precision medicine – the Orbitrap Exploris 240 mass spectrometer for large-scale plasma protein profiling

Michelle L Dubuke1 , Sitara Chauhan1 , Sarah Trusiak1 , and Emily I. Chen1APPLICATION NOTE 65952To demonstrate the reliability and robustness of the Thermo Scientific™ Orbitrap Exploris™ 240 mass spectrometer for large-scale, untargeted plasma protei...

Multi-pathway signaling analysis using a synthetic phosphopeptide panel, standardized sample preparation kits and SureQuant internal standard targeted quantitation

Aaron S. Gajadhar1, Bhavin Patel2, Penny Jensen2, Sebastien Gallien3,4, Romain Huguet1, Kay Opperman2, John C Rogers2, Andreas Huhmer1, Daniel Lopez-Ferrer1 International Hupo 2020To develop a universal phosphopeptide enrichment and internal standard...

Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction

Dounia Djeghloul, Bhavik Patel, Holger Kramer, Andrew Dimond, Chad Whilding, Karen Brown, Anne-Céline Kohler, Amelie Feytout, Nicolas Veland, James Elliott, Tanmay A. M. Bharat, Abul K. Tarafder, Jan Löwe, Bee L. Ng, Ya Guo, Jacky Guy, Miles K. Husey...

An Ultra High-throughput Plasma Protein Profiling (uHTPPP) Workflow Using a Modified Quadrupole-Orbitrap Mass Spectrometer

Michelle L. Dubuke1, Sarah Trusiak1, Sitara Chauhan1,Ryan D. Bomgarden2, Sergei Snovida2, Bhavin Patel2, Emily I. Chen1.An automated plasma protein profiling workflow on the new Orbitrap Exploris 240 mass spectrometer can deliver the consistency, sen...

Detecting Stable Isotope Labeled Metabolites in Untargeted Analysis Using a Benchtop Orbitrap Mass Spectrometer and a Single Processing Software Platform

Amanda Souza, Ioanna Ntai, Tatjana Talamantes and Ralf TautenhahnPurpose: Develop an integrated mass spectrometry-based workflow for stable isotope labeling (SIL) in untargeted metabolomics covering data acquisition and analysis in a single experimen...

Turnkey, Multi-pathway Signaling Analysis Using a Synthetic Phosphopeptide Panel, Standardized Sample Preparation Kits and SureQuant Internal Standard Targeted Quantitation

Aaron S. Gajadhar1; Bhavin Patel2; Penny Jensen2; Sebastien Gallien3,4; Romain Huguet1; Kay Opperman2; John C Rogers2; Andreas Huhmer1; Daniel Lopez-Ferrer1 ASMS 2020Purpose: We sought to develop a universal phosphopeptide enrichment and internal sta...

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