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Knowledge Base Articles

Expanding the Depth and Sensitivity of Cross-Link Identification by Differential Ion Mobility Using High-Field Asymmetric Waveform Ion Mobility Spectrometry

Lennart Schnirch, Michal Nadler-Holly, Siang-Wun Siao, Christian K. Frese, Rosa Viner, and Fan LiuIn cross-linking mass spectrometry (XL-MS), the depth and sensitivity of cross-link detection is often limited by the low abundance of cross-links compa...

Top-down analysis of immunoglobulin G isotypes 1 and 2 with electron transfer dissociation on a high-field Orbitrap mass spectrometer

Luca Fornelli a,1 , Daniel Ayoub a,2 , Konstantin Aizikov b , Xiaowen Liu c,d , Eugen Damoc b , Pavel A. Pevzner e , Alexander Makarov b , Alain Beck f , Yury O. Tsybin a,g, ⁎Journal of Proteomics Available online 27 February 2017The increasing impor...

Discovery of Novel Antimicrobial Peptides from Varanus komodoensis (Komodo Dragon) by Large-Scale Analyses and De-Novo-Assisted Sequencing Using Electron-Transfer Dissociation Mass Spectrometry

Barney M. Bishop*† , Melanie L. Juba†, Paul S. Russo‡, Megan Devine†, Stephanie M. Barksdale§, Shaylyn Scott†, Robert Settlage∥, Pawel Michalak⊥, Kajal Gupta#, Kent Vliet▽, Joel M. Schnur#, and Monique L. van Hoek§J. Proteome Res., Article ASAPKomodo...

SelectScience Interview: Mass Spectrometry Inventor, Alexander Makarov, Explains How State-of-the-Art MS is Being Utilized in World Leading Anti-Doping Laboratories

Alexander MakarovSelectScience InterviewThe Hong Kong Jockey Club is one of the largest and most well respected flat racing organizations in the world. The Club has a world class drug-testing facility, on site at the Sha Tin Racecourse. This internat...

How to do TMT experiments on Orbitrap based mass spectrometers. From sample preparation to data acquisition and data processing.

Xiaoyue Jiang and Rosa Viner A presentation providing an overview of multiplexed isobaric labeling, the basics of Tandem Mass Tags and the SPS MS3 technique. The presentation also covers sample prep. Specifically summary of the steps involved for a c...

Triomics Analysis of Imatinib-Treated Myeloma Cells Connects Kinase Inhibition to RNA Processing and Decreased Lipid Biosynthesis

Susanne B. Breitkopf, Min Yuan, Katja P. Helenius, Costas A. Lyssiotis and John M. AsaraAnalytical Chemistry, 2015, 87 (21), pp 10995–11006The combination of metabolomics, lipidomics, and phosphoproteomics that incorporates triple stable isotope la...

Performing nanoLC doesn’t have to be wicked hard

Southwick KiOrbi 2014 Tips on key practices that will help make you successful with nLC. Information on nLC and column maintenance as well as suggestions to increase the robustness of nLC.http://iconference.adobeconnect.com/p6m9b0mgxed/Thermo Fisher ...

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