Scientific Library

In-depth qualitative and quantitative analysis of composite glycosylation profiles and other micro-heterogeneity on intact monoclonal antibodies by high-resolution native mass spectrometry using a modified Orbitrap.

Rosati S, van den Bremer ET, Schuurman J, Parren PW, Kamerling JP, Heck AJ.MAbs. 2013 Aug 28;5(6). Here, we describe a fast, easy-to-use, and sensitive method to profile in-depth structural micro-heterogeneity, including intricate N-glycosylation pro...

Enhancing Mass Spectrometry for Biopharmaceutical Product Development

Horn D.WebinarMass spectrometry (MS) has proven to be an indispensable tool for developing complex biotherapeutics such as monoclonal antibodies. The ability to thoroughly characterize proteins facilitates investigation of the functional impact of po...

Monoclonal antibody cocktail as an enrichment tool for acetylome analysis

Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.Anal Chem. 2011 May 15;83(10):3623-6. The availability and robustness of methods to analyze phosphorylated proteins has greatly expanded our knowledge of phosphorylation based cell signaling. A key...

Mass measurement and top-down HPLC/MS analysis of intact monoclonal antibodies on a hybrid linear quadrupole ion trap-Orbitrap mass spectrometer

Bondarenko PV, Second TP, Zabrouskov V, Makarov A, Zhang Z.J Am Soc Mass Spectrom. 2009 Aug;20(8):1415-24Mass and top-down analyses of 150-kDa monoclonal immunoglobulin gamma (IgG) antibodies were performed on an Orbitrap analyzer. Three different sa...

Applications of mass spectrometry for the structural characterization of recombinant protein pharmaceuticals

Srebalus Barnes CA, Lim A.Mass Spectrom Rev. 2007 May-Jun;26(3):370-88.Therapeutic proteins produced using recombinant DNA technologies are generally complex, heterogeneous, and subject to a variety of enzymatic or chemical modifications during expre...