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Knowledge Base Articles

Deep Proteome Coverage and Label-Free Proteomic Analysis of Low Numbers of Mammalian Cells with a Quadrupole-Ion trap-Orbitrap Mass Spectrometer

Min Huang, Xiujie Sun, and Yue ZhouASMS 2020Purpose: To improve the proteome coverage and label-free quantitation performance of limited number of mammalian cells. Methods: Different sample preparation methods and LC-MS parameters were compared for p...

Standardized and scalable plasma protein quantitative profiling workflow using human blood based on a Thermo Scientific Q exactive platform

Jing Wang1; Sarah Trusiak1; Ryan D. Bomgarden2; Sergei Snovida2; Michelle Dubuke1, Emily I. Chen1APLICATION NOTE No. 73194To develop a high throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a s...

Standardized and scalable plasma protein quantitative profiling workflow using human blood based on a Thermo Scientific Q exactive platform

Jing Wang1; Sarah Trusiak1; Ryan D. Bomgarden2; Sergei Snovida2; Michelle Dubuke1, Emily I. Chen1APLICATION NOTE No. 73194To develop a high throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a s...

Ultra-sensitive LC/MS workflow for in-depth label-free analysis of single mammalian cells with nanodroplet sample processing

Khatereh Motamedchaboki1, Yongzheng Cong2, Yiran Liang2, Romain Huguet1, Yufeng Shen3, Xuefei Sun1, Greg Foster1, Daniel Lopez-Ferrer1, Andreas F. Huhmer1, Ying Zhu4 and Ryan T. Kelly2,4Hupo 2019In the last decade, single cell RNA sequencing has adva...

Powerful, standardized and versatile low-flow LCMS platform for deep dive proteomics and high-throughput analysis with maximum MS utilization

Oleksandr Boychenko1, Christopher Pynn1, Mike Baynham2, Jenny Ho3, Tabiwang Arrey4, and Peter Jehle1Hupo 2019Purpose: The availability and ease of sampling of blood and its constituents make it ideal for large cohort clinical research studies. Protei...

Standardized and scalable plasma protein quantitative profiling workflow using human blood based on a Thermo Scientific Q exactive platform

Jing Wang1; Sarah Trusiak1; Ryan D. Bomgarden2; Sergei Snovida2; Michelle Dubuke1, Emily I. Chen1APLICATION NOTE No. 73194To develop a high throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a s...

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