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Knowledge Base Articles

Expanding the Depth and Sensitivity of Cross-Link Identification by Differential Ion Mobility Using High-Field Asymmetric Waveform Ion Mobility Spectrometry

Lennart Schnirch, Michal Nadler-Holly, Siang-Wun Siao, Christian K. Frese, Rosa Viner, and Fan LiuIn cross-linking mass spectrometry (XL-MS), the depth and sensitivity of cross-link detection is often limited by the low abundance of cross-links compa...

Pushing the leading edge in protein analysis: Thermo Scientific Q Exactive HF-X hybrid quadrupole-Orbitrap mass spectrometer

BrochureUnderstanding biomolecules as drug targets, disease markers, and therapeutic agents demands highly sensitive analysis of complex biological samples, without sacrificing robustness and speed. The Thermo Scientific™ Q Exactive™ HF-X hybrid quad...

Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer - The Most Powerful Instrument Designed to Advance Your Scientific Pursuits

Orbitrap Fusion Lumos MS BrochurePUSHING NEW FRONTIERS IN SCIENCE Breakthroughs in science rely on great scientists making rapid progress answering challenging questions. “How does lipid metabolism affect cancer?” “How do non-cancerous cells mutate?”...

Dissociation behavior of a bifunctional tempoactive ester reagent for peptide structure analysis by free radical initiated peptide sequencing (FRIPS) mass spectrometry

Ihling C, Falvo F, Kratochvil I, Sinz A, Schäfer M.J. Mass Spectrom. 2015, 50, 396–406We have synthesized a homobifunctional active ester cross-linking reagent containing a TEMPO (2,2,6,6-tetramethylpiperidine-1-oxy) moiety connected to a benzyl gr...

A mechanism for actin filament severing by malaria parasite actin depolymerizing factor 1 via a low affinity binding interface.

Wong W, Webb AI, Olshina MA, Infusini G, Tan YH, Hanssen E, Catimel B, Suarez C, Condron M, Angrisano F, Nebi T, Kovar DR, Baum J.J Biol Chem. 2014 Feb 14;289(7):4043-54. doi: 10.1074/jbc.M113.523365. Epub 2013 Dec 26.Actin depolymerizing factor (ADF...

Structural characterization by cross-linking reveals the detailed architecture of a coatomer-related heptameric module from the nuclear pore complex

Shi Y, Fernandez-Martinez J, Tjioe E, Pellarin R, Kim SJ, Williams R, Schneidman D, Sali A, Rout MP, Chait BT.Mol Cell Proteomics. 2014 Aug 26. pii: mcp.M114.041673.Most cellular processes are orchestrated by macromolecular complexes. However, struct...

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