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Knowledge Base Articles

Sensitive and High-throughput Single-cell Proteomics Workflow on New Quadrupole-ion trap-Orbitrap Mass Spectrometer with FAIMS Separation

Khatereh Motamedchaboki1; Maowei Dou1; Yongzheng Cong2; Romain Huguet1; Aaron M Robitaille1; David M Horn1; Yufeng Shen3; Daniel Lopez-Ferrer1; Ryan T Kelly2, 4; Ying Zhu4.ASMS 2020Traditionally, proteomics experiments are applied to large population...

Pushing the Limits of Sensitivity: Micropillar Array-Based Chromatography Coupled to a Quadrupole Orbitrap Mass Spectrometer and FAIMS for Low-Input Proteomics

Julia Kraegenbring1, Karel Stejskal2, Claudia Ctortecka2, Gabriela Krššáková2, Jeff Op De Beeck3, Tabiwang N. Arrey1, Bernard Delanghe1, Alexander Harder1, Karl Mechtler4ASMS 20201 Thermo Fisher Scientific, Hanna-Kunath-Str. 11, Bremen, Germany. 2 In...

High-throughput single cell proteomics analysis with nanodroplet sample processing, multiplex TMT labeling, and ultra-sensitive LC-MS

Khatereh Motamedchaboki1 , Maowei Dou1 , Yongzheng Cong2 , Romain Huguet1 , Aaron M Robitaille1 , Yufeng Shen3 , Geremy Clair4 ,Charles Ansong4 , Daniel Lopez-Ferrer1 , Ryan T. Kelly2,4, and Ying Zhu4APPLICATION NOTE 65714Understanding heterogeneity ...

High-Throughput Single Cell Proteomics Analysis with Nanodroplet Sample Processing, Multiplex TMT labeling, and Ultra-Sensitive LC-MS

Khatereh Motamedchaboki1, Maowei Dou1, Yongzheng Cong2, Romain Huguet1, Aaron M Robitaille1, Yufeng Shen3, Geremy Clair,4 Charles Ansong,4 Daniel Lopez-Ferrer1, Ryan T. Kelly2,4, and Ying Zhu4 Hupo 2019Understanding heterogeneity at single cell level...

Ultra-sensitive LC/MS workflow for in-depth label-free analysis of single mammalian cells with nanodroplet sample processing

Khatereh Motamedchaboki1, Yongzheng Cong2, Yiran Liang2, Romain Huguet1, Yufeng Shen3, Xuefei Sun1, Greg Foster1, Daniel Lopez-Ferrer1, Andreas F. Huhmer1, Ying Zhu4 and Ryan T. Kelly2,4Hupo 2019In the last decade, single cell RNA sequencing has adva...

Metabolomic Analysis of 13C/15N Labeled Metabolites Using High Resolution Orbitrap Mass Spectrometry and Compound Discoverer Software

Anastasia Kalli1, Bryson Bennett2, Junhua Wang1, Caroline Ding1 and Ralf Tautenhahn1Utilize ultra-high resolution mass spectrometry for resolving 13C and 15N double-labeled metabolites extracted from MDB-MA-231 cells Perform metabolite ID with Thermo...

Capturing States of Pluripotency: How Cell Surface Chemoproteomics Facilitates Rapid Immunophenotyping and Sorting of Pluripotent Stem Cells

Gundry R.WebinarInduction of a pluripotent state in somatic cells through nuclear reprogramming has ushered in a new era of regenerative medicine. However, heterogeneity and varied differentiation potentials among induced pluripotent stem cell (iPSC)...

Breaking the 2000-Protein Barrier in a Standard LC Run Using a New Orbitrap Benchtop Instrument and Multiple Search Engines

Paschke C, Kellmann M, Xuan Y, Damoc E, Ueckert T, Grensemann H, Comberg U, Delanghe B.Scientific PosterGetting a comprehensive view of the protein content of a complex sample is still a challenge in proteomics; accomplishing it in one analytical run...

Evaluating the potential of a novel oral lesion exudate collection method coupled with mass spectrometry-based proteomics for oral cancer biomarker discovery

Kooren JA, Rhodus NL, Tang C, Jagtap PD, Horrigan BJ, Griffin TJ.Clin Proteomics. 2011 Sep 13;8:13.Early diagnosis of Oral Squamous Cell Carcinoma (OSCC) increases the survival rate of oral cancer. For early diagnosis, molecular biomarkers contained ...

A large network of interconnected signaling pathways in human ovarian follicles is supported by the gene expression activity of the granulosa cells

Yoo SW, Savchev S, Sergott L, Rezai T, Lopez MF, Von Wald T, Eaton JL, Reindollar R, Usheva A.Reprod Sci. 2011 May;18(5):476-84.Human follicular fluid (hFF), as an extra oocyte microenvironment, is essential to the biological processes of oocyte deve...

Evaluation of the deuterium isotope effect in zwitterionic hydrophilic interaction liquid chromatography separations for implementation in a quantitative proteomic approach

Di Palma S, Raijmakers R, Heck AJ, Mohammed S.Anal Chem. 2011 Nov 1;83(21):8352-6. Quantitative methodologies for the global in-depth comparison of proteomes are frequently based on chemical derivatization of peptides with isotopically distinguishabl...

A simple, automated, high resolution mass spectrometry method to determine the disulfide bond and glycosylation patterns of a complex protein: the subgroup a avian sarcoma and leukosis virus envelope glycoprotein

Pike GM, Madden BJ, Melder DC, Charlesworth MC, Federspiel MJ.J Biol Chem. 2011 May 20;286(20):17954-67. Enveloped viruses must fuse the viral and cellular membranes to enter the cell. Understanding how viral fusion proteins mediate entry will provid...

Quantitative N-linked glycoproteomics of myocardial ischemia / reperfusion injury reveals early remodeling in the extracellular environment

Parker BL, Palmisano G, Edwards AV, White MY, Engholm-Keller K, Lee A, Scott NE, Kolarich D, Hambly BD, Packer NH, Larsen MR, Cordwell SJ.Mol Cell Proteomics. 2011 Aug;10(8):M110.006833.Extracellular and cell surface proteins are generally modified w...

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