Scientific Library

Thermo Scientific Orbitrap Eclipse Tribrid Mass Spectrometer

Orbitrap Eclipse BrochureGo beyond today’s discovery When complex analytical questions require a definitive answer, you need a powerful and versatile solution that will allow you to accurately resolve subtle differences, distinguish the right answer ...

Thermo Scientific Orbitrap MS Workshop: Smarter. Faster. Simpler. Becoming an Orbitrap Power User.

Thermo Scientific and Customer PresentersASMS 2019 Orbitrap Power User MeetingExcited by the power and potential of your Thermo Scientific Orbitrap-based mass spectrometer? From small molecules to large, receive training on system calibration and mai...

Advanced Peak Determination (APD) Method Construction Guide and Downloadable Method

Romain Huguet, Helene Cardasis, Shannon Eliuk, Vlad Zabrouskov, Graeme McAlister.Method GuideThe new APD feature requires method optimization for optimal results. Here we provide a downloadable method, optimized for 1 microgram of tryptic HeLa digest...

Thermo Scientific Q Exactive HF-X Hybrid Quadrupole-Orbitrap MS System: Product Specification

Product Specification• Scan speed up to 40 Hz with ultra-high-field Thermo Scientific™ Orbitrap™ mass analyzer technology • Resolution of up to 240,000 (FWHM) at m/z 200 • High Capacity Transfer Tube (HCTT) and electrodynamic ion funnel for improved ...

Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer with Advanced Peak Determination

APD BrochureReading Between the Lines with Advanced Peak Determination High performance mass spectrometers with extreme sensitivity and resolution have illuminated a need for improved on-the-fly spectral analysis and peak assignment. The Advanced Pea...

Maximizing proteome coverage through improved on-line Orbitrap peak determination

Graeme C. McAlister1, Christian Thoeing2, Helene L. Cardasis1, Alex S. Hebert3, Romain Huguet1, Philip Remeš1, Andreas Kuehn2, Mike Senko1, Shannon Eliuk1, Joshua J. Coon3, Vlad Zabrouskov1ASMS 2017 PosterThermo Scientific™ Tribrid™ mass spectrometer...

Pushing the leading edge in protein analysis: Thermo Scientific Q Exactive HF-X hybrid quadrupole-Orbitrap mass spectrometer

BrochureUnderstanding biomolecules as drug targets, disease markers, and therapeutic agents demands highly sensitive analysis of complex biological samples, without sacrificing robustness and speed. The Thermo Scientific™ Q Exactive™ HF-X hybrid quad...

Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer - The Most Powerful Instrument Designed to Advance Your Scientific Pursuits

Orbitrap Fusion Lumos MS BrochurePUSHING NEW FRONTIERS IN SCIENCE Breakthroughs in science rely on great scientists making rapid progress answering challenging questions. “How does lipid metabolism affect cancer?” “How do non-cancerous cells mutate?”...

Q Exactive BioPharma: The complete BioPharma characterization solution

Q Exactive BioPharma BrochureThe Thermo Scientific™ Q Exactive™ BioPharma platform offers distinct operational modes that have been optimized for the top 3 protein characterization workflows: • Denatured and native MS intact analysis with the new Hig...

Full Characterization of Heterogeneous Antibody Samples Under Denaturing and Native Conditions on a Hybrid Quadrupole-Orbitrap Mass Spectrometer

Scheffler K, Damoc E, Sutton J, Bailey A, Houel S, Josephs J.ASMS 2016 PosterHere we cover the three major workflows for characterization of biopharmaceuticals on one single instrument LC-MS platform using single and mixed antibody samples: 1)intact ...

Complete Characterization of a Cysteine-linked Antibody-Drug Conjugate Performed on a Hybrid Quadrupole-Orbitrap Mass Spectrometer with High Mass Range

Bailey A, Damoc E, Houel S, Scheffler K, Josephs J. ASMS 2016 PosterWe have modified the instrument control software of a benchtop quadrupole-Orbitrap mass spectrometer to add native MS capability. In this study we demonstrate complete characterizati...

A Journey in Biotherapeutic Peptide Mapping

Cheng G C. https://www.youtube.com/watch?v=boL6vZ4s4hkLearn about how the latest chromatography, mass spectrometry and software are accelerating confident decision making at Alexion Pharmaceuticals, Inc. Discover how this biopharmaceutical company, f...

Factory-certified program: Access technology you only dreamed possible with factory-certified mass spectrometers

Now you can get the mass spectrometer of your dreams, at a low cost, for any qualitative or quantitative workflow. When purchasing directly from Thermo Fisher Scientific you will not only maintain your competitive edge but will have peace of mind you...

Thermo Scientific™ Nano LC Trap Column Installation Guide for Mass Spectrometric Analysis

Thermo Scientific Center of ExcellenceTrap Column Installation GuideThis technical guide is an in-depth description of how to install an online Trap column on nano LC systems including EASY-nLC 1200, EASY-nLC 1000, UltiMate 3000 RSLCnano, and EASY-nL...

De novo sequencing and resurrection of a human astrovirus-neutralizing antibody

Walter A. Bogdanoff, David Morgenstern, Marshall Bern, Beatrix M. Ueberheide, Alicia Sanchez-Fauquier, and Rebecca M. DuBoisACS Infect. Dis., Just AcceptedMonoclonal antibody (mAb) therapeutics targeting cancer, autoimmune diseases, inflammatory dise...

New integrated informatics solution for protein biotherapeutics characterization

Stephane Houel, Jennifer Sutton, Terry Zhang, Jonathan Josephs, Mark SandersPoster Note 72101Integrated informatics solution for protein biotherapeutics characterization. Two software packages Thermo Scientific™ Protein Deconvolution and Thermo Scien...

Targeted nano LC-MS Quantification of Rituximab in Human Bio-Matrix with High-Resolution Accurate MS

A. Boychenko, M Samonig, F Steiner, R SwartThermo Fisher Scientific, Germering, Germany

Analysis of monoclonal antibody sequence and post-translational modifications by time-controlled proteolysis and tandem mass spectrometry

Lichao Zhang1, A. Michelle English1, Dina L. Bai1, Scott A. Ugrin1, Jeffrey Shabanowitz1, Mark M. Ross1, Donald F. Hunt1,2,* and Wei-Han Wang1Mol Cell Proteomics. 2015 Nov 29. pii: mcp.O115.056721Methodology for sequence analysis of ~150 kDa monoclon...

High-Resolution, Accurate-Mass Orbitrap Mass Spectrometry – Definitions, Opportunities, and Advantages

Kerstin Strupat, Olaf Scheibner, and Maciej Bromirski This Technical Note considers and applies the latest IUPAC recommendations regarding terms—mass resolving power, mass resolution, mass accuracy, and mass precision. With Orbitrap technology, true ...

Characterization of Monoclonal Antibodies and ADCs using a Benchtop Orbitrap Mass Spectrometer

Zheng X.ASMS 2015 Poster Note 64397Purpose: To characterize mAbs and ADCs with high resolution Orbitrap mass spectrometer. Methods: Coupling High Performance Liquid Chromatography with high resolution Orbitrap mass spectrometer (HPLC-MS) enables accu...

Orbitrap Fusion MS for Glycan and Glycopeptide Analysis

Saba J.White Paper 64385This document is ideal for scientists who are writing grants and would like to learn what Orbitrap Fusion MS can do for Glycomics and Glycoproteomics. The document showcases novel workflows and acquisition strategies that are ...

Thermo Scientific Orbitrap Fusion Tribrid MS: Overview

Overview Presentation: Updated November 2014Orbitrap Fusion Tribrid MS is the ultimate research tool for those facing the most difficult analytical challenges in cell biology, proteomics, and structural analysis. This slide presentation shows represe...

A “Universal” data-dependent mass spectrometry method that eliminates time-consuming method optimization for achieving maximal identifications from each sample

Eliuk S, Soltero N, Remes P, Scheffler K, Senko M, Zabrouskov V.2014 Poster and method for software version 1.1 and 1.2 SP1***Updated 4/15/15 with new instrument method for new software release. Developing optimal methods for samples, particularly th...

Comprehensive Sequence and Post-translational Modifications Analysis of Monoclonal Antibody by Flash Digest and LC-High Resolution MS

Wang HJ, O'Grady J, Horn D, Hao Z, Meyer K, Josephs J.ASMS 2014 PosterPurpose: To develop a workflow for fast and comprehensive characterization of peptide sequence, identification and relative quantitation of post-translational modifications (PTMs) ...

Improving Intact Antibody Characterization by Orbitrap Mass Spectrometry

Scheffler K, Damoc E, Muller M, Zeller M, Moehring TBioPharma_PN63712_E 11/13SHere we present an approach to analyze an intact monoclonal antibody in non-reduced and reduced condition by LC-MS using the Thermo Scientific™ Orbitrap Elite™ mass spectro...

Comprehensive Comparison of Biosimilar Protein Drugs by High-Separation and High-Resolution LC-MS

Horn D, Wu SL, Zhang F, Viner R, Hao Z.ASMS 2013 Oral Presentationhttp://www.youtube.com/watch?v=rkjBb1STLm8Thermo Fisher Scientific

Identification of isomerization and racemization of aspartate in the Asp–Asp motifs of a therapeutic protein

Zhang J, Yip H, Katta V.Anal Biochem. 2011 Mar 15;410(2):234-43.A thermally stressed Fab molecule showed a significant increase of basic variants in imaged capillary isoelectric focusing (iCIEF) analysis. Mass analyses of the reduced protein found an...

Rapid identification of low level glycation sites in recombinant antibodies by isotopic labeling with 13C6-reducing sugars

Zhang J, Zhang T, Jiang L, Hewitt D, Huang Y, Kao YH, Katta V.Anal Chem. 2012 Mar 6;84(5):2313-20.Recombinant antibodies exhibit low levels of glycation from exposure to reducing sugars during production. As the glycation sites are typically distribu...

Structural characterization of intact antibodies by high-resolution LTQ Orbitrap mass spectrometry

Zhang J, Liu H, Katta V.J Mass Spectrom. 2010 Jan;45(1):112-20.Recombinant monoclonal antibodies (MAbs) can be heterogeneous due to modifications that can occur during expression, purification or during storage. These large multichain proteins (appro...

Application of liquid chromatography-mass spectrometry in the study of metabolic profiling of cirrhosis in different grades

Du Z, Zhang L, Liu S.Se Pu. 2011 Apr;29(4):314-9.The metabolic profiles were obtained by high performance liquid chromatography combined with a LTQ Orbitrap XL mass spectrometer (HPLC-LTQ Orbitrap XL MS) platform to analyze serum specimens from the p...

AnalyteGuru

Knowledge Base Articles