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Knowledge Base Articles

Antibody subunit analysis workflow on a quadrupole-Orbitrap mass spectrometer: from optimized sample preparation to data analysis

1. Kai Scheffler 2. Eugen DamocAPPLICATION NOTE 72854The goal of this application note is to discuss the importance of proper sample preparation for mAb subunit analysis and to provide optimized LC/MS conditions to achieve conclusive information on t...

Thermo Scientific Q Exactive UHMR Hybrid Quadrupole-Orbitrap mass spectrometer: Go beyond what you thought possible with native MS

The Thermo Scientific™ Q Exactive™ UHMR Hybrid Quadrupole-Orbitrap™ mass spectrometer is the first to provide the unique combination of unprecedented ultra-high mass range resolution, highest sensitivity, and MS2 and pseudo-MS3 capabilities needed to...

Complete characterization of a lysine-linked antibody drug conjugate by native LC/MS intact mass analysis and peptide mapping

Aaron O. Bailey,1 Stephane Houel,1 Kai Scheffler,2 Eugen Damoc,3 Jennifer Sutton,1 Jonathan L. JosephsAPPLICATION NOTE 72511To perform complete characterization of the lysine-linked antibody drug conjugate (ADC) trastuzumab emtansine using both intac...

Full characterization of heterogeneous antibody samples under denaturing and native conditions on the Q Exactive BioPharma mass spectrometer

Kai Scheffler and Eugen DamocTo demonstrate the capabilities of the Thermo Scientific™ Q Exactive™ BioPharma mass spectrometry platform, particularly the High Mass Range (HMR) Mode for the characterization of antibody samples. The three different ope...

Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer with UVPD: Designed to Fragment the Most Challenging Compounds

New Opportunities for the Characterization of Intact Monoclonal Antibodies Top-down and middle-down mass spectrometry offer important advantages for the characterization of biotherapeutics with complete molecular specificity. Attaining comprehensive ...

Pushing the leading edge in protein analysis: Thermo Scientific Q Exactive HF-X hybrid quadrupole-Orbitrap mass spectrometer

BrochureUnderstanding biomolecules as drug targets, disease markers, and therapeutic agents demands highly sensitive analysis of complex biological samples, without sacrificing robustness and speed. The Thermo Scientific™ Q Exactive™ HF-X hybrid quad...

A robust and sensitive capillary flow LC – high-resolution accurate-mass MS platform for discovery and targeted proteomics

Alexander Boychenko1, Mike Baynham2, Stephan Meding1; Martin Ruehl1; Martin Samonig1; Remco Swart1International Hupo 2016We systematically investigated the effect of flow rate and column ID on MS sensitivity using samples with different complexity. T...

Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer - The Most Powerful Instrument Designed to Advance Your Scientific Pursuits

Orbitrap Fusion Lumos MS BrochurePUSHING NEW FRONTIERS IN SCIENCE Breakthroughs in science rely on great scientists making rapid progress answering challenging questions. “How does lipid metabolism affect cancer?” “How do non-cancerous cells mutate?”...

A Journey in Biotherapeutic Peptide Mapping

Cheng G C. https://www.youtube.com/watch?v=boL6vZ4s4hkLearn about how the latest chromatography, mass spectrometry and software are accelerating confident decision making at Alexion Pharmaceuticals, Inc. Discover how this biopharmaceutical company, f...

PD Biomarker Measurement by LC/MS/MS – Resolving Sensitivity and Throughput Challenges Using High Resolution Mass Spectrometry

Sturm RM, Jones BR, Shan L, Buckholz JE, Biondolillo RF, Mulvana DETo implement the EASY-Spray nano-electrospray ion source (Thermo Scientific, Inc.) for quantification of beta nerve growth factor (β-NGF) in human serum by immunoprecipitation combine...

Development of LC-MS methods for quantitation of hepcidin and demonstration of siRNA-mediated hepcidin suppression in serum.

Li S, Nakayama T, Akinc A, Wu SL, Karger BL.J Pharmacol Toxicol Methods. 2014 Oct 2. pii: S1056-8719(14)00266-4. doi: 10.1016/j.vascn.2014.09.008.A requisite step in developing a therapeutic to modulate the levels of hepcidin is the development of a ...

In-depth qualitative and quantitative analysis of composite glycosylation profiles and other micro-heterogeneity on intact monoclonal antibodies by high-resolution native mass spectrometry using a modified Orbitrap.

Rosati S, van den Bremer ET, Schuurman J, Parren PW, Kamerling JP, Heck AJ.MAbs. 2013 Aug 28;5(6). Here, we describe a fast, easy-to-use, and sensitive method to profile in-depth structural micro-heterogeneity, including intricate N-glycosylation pro...

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