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Knowledge Base Articles

Native Top-Down Analysis of Intact Antibodies using Multiple Dissociation Techniques on a Tribrid Quadrupole Orbitrap Linear Ion Trap Mass Spectrometer

Eugen Damoc1, Kristina Srzentić2, Romain Huguet3, Graeme McAlister3, Christopher Mullen3, Philip M Remes3, Jesse D Canterbury3, Mike Senko3, and Vlad Zabrouskov3 ASMS 2019 PosterPurpose: Evaluate the performance of the new Thermo Scientific™ Orbitrap...

Antibody subunit analysis workflow on a quadrupole-Orbitrap mass spectrometer: from optimized sample preparation to data analysis

1. Kai Scheffler 2. Eugen DamocAPPLICATION NOTE 72854The goal of this application note is to discuss the importance of proper sample preparation for mAb subunit analysis and to provide optimized LC/MS conditions to achieve conclusive information on t...

Complete characterization of a lysine-linked antibody drug conjugate by native LC/MS intact mass analysis and peptide mapping

Aaron O. Bailey,1 Stephane Houel,1 Kai Scheffler,2 Eugen Damoc,3 Jennifer Sutton,1 Jonathan L. JosephsAPPLICATION NOTE 72511To perform complete characterization of the lysine-linked antibody drug conjugate (ADC) trastuzumab emtansine using both intac...

Full characterization of heterogeneous antibody samples under denaturing and native conditions on the Q Exactive BioPharma mass spectrometer

Kai Scheffler and Eugen DamocTo demonstrate the capabilities of the Thermo Scientific™ Q Exactive™ BioPharma mass spectrometry platform, particularly the High Mass Range (HMR) Mode for the characterization of antibody samples. The three different ope...

High Resolution LC/MS Separation and Characterization of Chemoenzymatic Site-specific Engineered Antibody-Drug Conjugates

Shanhua Lin1, Terry Zhang2, Jonathan Josephs2, Brian Agnew3, and Xiaodong Liu1ASMS 2017 PosterPurpose: Demonstrate application of the SiteClick™ technology to the production of antibody-drug conjugates (ADCs) and a number of different detection probe...

Pushing the leading edge in protein analysis: Thermo Scientific Q Exactive HF-X hybrid quadrupole-Orbitrap mass spectrometer

BrochureUnderstanding biomolecules as drug targets, disease markers, and therapeutic agents demands highly sensitive analysis of complex biological samples, without sacrificing robustness and speed. The Thermo Scientific™ Q Exactive™ HF-X hybrid quad...

Precise quantification of mixtures of bispecific IgG produced in single host cells by liquid chromatography-Orbitrap high-resolution mass spectrometry

Yiyuan Yin, Guanghui Han, Jianhui Zhou, Michael Dillon, Luke McCarty, Lou Gavino, Diego Ellerman, Christoph Spiess, Wendy Sandoval & Paul J. Carter MABS 2016, VOL. 8, NO. 8, 1467–1476Bispecific IgG are heterotetramers comprising 2 pairs of heavy an...

Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer - The Most Powerful Instrument Designed to Advance Your Scientific Pursuits

Orbitrap Fusion Lumos MS BrochurePUSHING NEW FRONTIERS IN SCIENCE Breakthroughs in science rely on great scientists making rapid progress answering challenging questions. “How does lipid metabolism affect cancer?” “How do non-cancerous cells mutate?”...

Enabling Mass Spectrometric Analysis of Intact Proteins in Native Conditions on A Hybrid Quadrupole-Orbitrap Mass Spectrometer

Scheffler K, Damoc E, Bailey A, Josephs J.ASMS 2016 PosterWe have successfully implemented the High Mass Range Mode on the Q Exactive Plus and Q Exactive HF mass spectrometers allowing for mass detection up to m/z 8000. This new operating mode extend...

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