irm-LC/MS: δ13C Analysis of Underivatized Amino Acids
Dieter Juchelka, Michael Krummen
Thermo Fisher Scientific, Bremen, Germany
AN30065
Isotope ratio monitoring-LC/MS (irm-LC/MS) with the Thermo Scientific LC IsoLink allows determination of 13C/12C ratios of individual amino acids without derivatization, eliminating all of the possible errors, limitations, and time requirements of derivatization. For amino acids water is the best solvent; HPLC columns are available on which the separation of amino acids can be performed with an aqueous mobile phase. The LC IsoLink offers new access to 13C-tracer experiments which investigate amino acid and protein metabolism.
- IRMS
- HPLC
- Carbon isotopes
- Amino acids
United States
