irm-GC/MS: δ13C in Fatty Acid Methyl Esters (FAME)

[claudia-bouman]

irm-GC/MS: δ13C in Fatty Acid Methyl Esters (FAME)
Andreas W. Hilkert (1), Dieter Juchelka (1) and Charles B. Douthitt (2)
1) Thermo Fisher Scientific, Bremen, Germany, 2) Thermo Fisher Scientific, Arizona, USA
AN30052
Isotope Ratio Monitoring GC/MS (irm-GC/MS) measurement of the delta13C content of the methyl esters of fatty acids (FAME) has been used to study natural food webs and, with the use of highly delta13C –enriched precursors, fatty acid metabolism in vivo. The use of biolabeled fatty acids to study metabolism is a relatively new technique which combines tracer methodology with natural abundance measurements by using naturally labeled compounds as tracers.

• GC-IRMS
• Gas Chromatography
• Fatty Acid Methyl Esters