Variable Data-Independent Acquisition (vDIA) Delivers High Selectivity and Sensitivity in Combined Targeted and Untargeted Analyses for Small Molecules

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Scheibner O, Kellmann M, Yang C, Bromirski M ,
TN-64283-LC-Veterinary-Drug-Residues-Muscle-Kidney-Milk-Plasma-TN64283
Goal: Forty-four multi-class veterinary drugs of known concentrations were employed to demonstrate how the use of a generic variable data independent acquisition (vDIA) method with wide MS/MS precursor isolation windows achieves sensitivity and selectivity comparable to data-dependent MS2 acquisition (using narrow isolation windows) in quantitative and qualitative small molecule applications. In addition, a full record of MS and MS/MS data for the measured sample fit for non-targeted and unknown screening purposes is delivered.


Thermo Fisher Scientific