on 05-11-201704:42 AM - edited on 11-09-202104:00 AM by usermigration2
1) Erasmus Medical Center – Sophia Children's Hospital, Department of Paediatrics, Division of Neonatology, PO Box 2040, 3000 CA Rotterdam, The Netherlands, 2) Intellectual Disability Medicine, Department of General Practice, Erasmus MC, Rotterdam, The Netherlands, 3) Department of Paediatric Surgery, Erasmus MC – Sophia, Rotterdam, The Netherlands Rapid Communications in Mass Spectrometry (2009), V23 (22), pp3549–3554, doi: 10.1002/rcm.4279 Validation of deuterium and oxygen18 in urine and saliva samples from children using on-line continuous-flow isotope ratio mass spectrometry H. Schierbeek (1), R. Rieken (2,3), K. Y. Dorst (1), C. Penning (2), J. B. van Goudoever (1) The doubly labelled water method is valuable for measuring energy expenditure in humans. It usually involves blood or urine sampling, which might be difficult in neonates and children with cerebral palsy or other disabilities. We therefore aimed to validate a method making use of saliva samples analyzed by automated thermal conversion elemental analyzer in combination with isotope ratio mass spectrometry (TC-EA/IRMS). The subjects received labelled water orally and urine and saliva samples were collected and analyzed. Deuterium as well as oxygen18 was measured in one single run using a peak jump method. Excellent linearity was found for measurement of enrichments of deuterium (R2 = 0.9999) and oxygen18 (R2 = 0.9999).