Wang L, Yang C, Jiang G. EPRW 2012 Poster Purpose: To develop a fast and sensitive screening and quantitative method for multi-residue (>300) pesticides analysis using ultra-high performance liquid chromatography and high resolution/accurate mass (UHPLC-HR/AM) platform. And to evaluate its performance for pesticides screening and quantitation in food and environmental matrices.
Methods: Chromatographic separation was achieved on an Thermo Scientific Dionex UltiMate 3000 RSLC UHPLC system with a Thermo Scientific Accucore aQ column. A Thermo Scientific Exactive Plus HR/AM mass spectrometer was operated in full scan or full scan/all ion fragmentation (AIF) scan mode for data acquisition.
Results: More than 300 pesticides were sufficiently retained and separated within 16 minutes. Screening for target pesticides was performed using Thermo Scientific ExactFinder data processing software with retention time used for identification, isotopic pattern and/or fragment ions used for confirmation. Quantitation was performed using Thermo Scientific TraceFinder data processing software. For selected representative pesticides, excellent quantitation performance was achieved with r2 >0.99 for most compounds, and accuracy ranges from 90.7% to 116% at 10 ppb with %RSD in the range 0.6% to 11%. The limit of quantitation (LOQ) was observed from 50 ppt to 1 ppb for most pesticides.