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Using HRAM Survey Analysis Combined with Rapid MS2 Data to Develop a Fragmentation Based Detection Workflow for Structure ID Acquisition

Reputable Mentor II
Reputable Mentor II
Stratton T, Ding C, Henrich C, Grensemann H, Pfaff H, Strohalm M, Kusch S.
ASMS 2013 Poster
Purpose: Demonstrate a workflow for metabolite detection and structure elucidation combining HRAM (high-resolution, accurate-mass) HCD MS2 with a new customizable processing workflow using orthogonal metabolite detection tools to direct multiple fragmentation mode MSn acquisition. Methods: Samples of human urine, post-kidney operation, were analyzed by rapid HRAM HCD MS2 for peak detection. Potential metabolite peaks were acquired by multiple-fragment-mechanism MSn for structure identification where an HCD MS2 scan was followed by multiple CID MS3 fragmentation scans. Results: Multiple metabolites for each of four co- and serially administered drugs commonly used during surgery were detected and identified.

Thermo Fisher Scientific
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Last update:
‎10-15-2021 11:28 AM
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