Stability, robustness and time efficiency are three major challenges in laboratories where a large number of samples are analyzed routinely. Here we report usability study of a new two channels HPLC, a new tandem mass spectrometer and a new data processing software to address these challenges. A total of 2,000 crashed synthetic serum samples spiked with Alprazolam and isotopic internal standard were analyzed continuously over 100 hours, with additional 44 QC samples. Instrument-to-instrument precision studies (3 units) are reported with four example compounds (Reserpine, Chloramphenicol, Testosterone, Estradiol) using both HESI probe and APCI probe in both positive and negative ionization modes.
United States
