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Untargeted Metabolomics Workflow Using UHPLC/Quadrupole Orbitrap Mass Spectrometer and SIEVE 2.1 Software

Reputable Mentor II
Reputable Mentor II
Wang J, Peake DA, Sanders M, Athanas M, Huang Y
ASMS 2013 Poster Note
Metabolomics is a rapidly growing field of post-genomic biology, aiming to comprehensively characterize the small molecules in biological systems. Nonbiological systematic biases from instrument calibration or the order of sample injection account for the most significant errors in LC/TOF-MS data [1]. Here we present a workflow using a UHPLC/benchtop quardrupole Orbitrap platform and automated data analysis software for untargeted metabolomic profiling of plasma samples for biomarker discovery from the Zucker diabetes fatty (ZDF) rat model. The optimal conditions for sample preparation, liquid chromatography (LC), column, mass spectrometry (MS), and data processing parameters are explored.

Thermo Fisher Scientific
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Last update:
‎10-15-2021 05:02 AM
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