on 05-01-201210:39 AM - edited on 10-15-202111:59 AM by AnalyteGuru
Wiśniewski JR, Zougman A, Nagaraj N, Mann M. Nat Methods. 2009 May;6(5):359-62. We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry–based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.