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Universal sample preparation method for proteome analysis

Reputable Mentor II
Reputable Mentor II
Wiśniewski JR, Zougman A, Nagaraj N, Mann M.
Nat Methods. 2009 May;6(5):359-62.
We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry–based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.
Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Martinsried, Germany.
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