Wiśniewski JR, Zougman A, Nagaraj N, Mann M.
Nat Methods. 2009 May;6(5):359-62.
We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry–based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.
http://www.nature.com/nmeth/journal/v6/n5/abs/nmeth.1322.htmlDepartment of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Martinsried, Germany.
United States
