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Ultra-sensitive LC/MS workflow for in-depth label-free analysis of single mammalian cells with nanodroplet sample processing

Reputable Mentor II
Reputable Mentor II
Khatereh Motamedchaboki1, Yongzheng Cong2, Yiran Liang2, Romain Huguet1, Yufeng Shen3, Xuefei Sun1, Greg Foster1, Daniel Lopez-Ferrer1, Andreas F. Huhmer1, Ying Zhu4 and Ryan T. Kelly2,4
Hupo 2019
In the last decade, single cell RNA sequencing has advanced our understanding of transcript heterogeneity. Currently, there are strong efforts to enable single cell proteomic analysis using mass spectrometry (MS)-based workflows. While the analysis of single-cell-sized aliquots from bulk-prepared tryptic digests has been demonstrated, only recently have label-free strategies been reported for profiling hundreds of proteins from single mammalian cells. Further development in sample processing, separations, MS and data analysis are necessary to realize single cell proteomics with greater depth of coverage and quantitative accuracy. Here we introduce an improved LC separation coupled to the new Thermo Scientific™ Orbitrap Eclipse™ Tribrid™ mass spectrometers with a Thermo Scientific™ FAIMS Pro™ interface to increase proteome coverage for single mammalian cells.

1 Thermo Fisher Scientific, San Jose, CA 2 Brigham Young University, Provo, UT 3 CoAnn Technologies LLC, Richland, WA 4 Pacific Northwest National Laboratory, Richland, WA, USA
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Last update:
‎10-15-2021 06:54 AM
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