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Ultra-Fast Analysis of Allergens Using Capillary Electrophoresis Coupled to Mass Spectrometry and UVPD

Reputable Mentor II
Reputable Mentor II
Chien-Hsun Chen1, Andreas Krupke1, Monica Carrera2, Romain Huguet1, Shiaw-Min Chen3, Achim Karger3, Steve Williams3, Michael Wenz3, Andreas FR Huhmer1, Aran Paulus1 and Daniel Lopez-Ferrer1
International Hupo 2016
The identification of proteins in food matrices that cause sensitization in individuals or allergic reactions in those individuals already sensitized, represent a major concern for the food industry. Here we explore the application of CE/MS/MS as an accurate and sensitive way to rapidly test for protein allergens in food. The workflow is based on a simple protein extraction step and the use of capillary electrophoresis (CE) hyphenated to a Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ mass spectrometer with an UVPD source for the characterization of the extracted proteins. The high mass accuracy and resolution, coupled to the efficient isoform separation and the different fragmentation modes allow for the rapid allergen identification and its origin.

1 Thermo Fisher Scientific, San Jose, USA 2 Marine Research Institute, Vigo, Spain 3 Thermo Fisher Scientific, South San Francisco, USA
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Last update:
‎10-15-2021 06:12 AM
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