Devin Drew1, Prasanna Rajagopal2, Michelle Puchowicz3, and Daniela Schlatzer3
ASMS 2017 Poster
Purpose: Investigate the extent of common and unique proteome, transcriptome, and pathway changes that occur in rat liver on controlled high fat diets.
Methods: Perform differential analysis on control diet vs. high fat diets using LC/MS/MS based proteome and microarray based transcriptome analyses.
Results: Proteome differential analysis highlights fatty acid metabolism and peroxisome pathways, now corroborated by Reactome and WikiPathways sources. Transcriptome analysis shows gastrin and leptin signaling, RAF/MAP kinase cascade among other pathways, and no overlap was found with pathways over-represented in our proteome results, possibly reflecting longer-term processes observed at the transcript level.
1. Thermo Fisher Scientific, San Jose, CA
2. Thermo Fisher Scientific, Bengaluru, KA, India
3. School of Medicine, Case Western Reserve University, Cleveland, OH, USA
United States
