on 06-19-201703:55 PM - edited on 10-15-202111:43 AM by AnalyteGuru
Sebastien Gallien1, Yue Xuan2, Shouling Xu3, Markus Kellmann2, Bhavin Patel4, John Rogers4, Alexander Harder2, Andreas Huhmer5, Ken Miller5 ASMS 2017 Poster Purpose: Evaluation of the performance of advanced parallel reaction monitoring (PRM) methods to monitor signaling pathways in cancer cell lines for clinical research.
Methods: A broad range of PRM methods (including internal standard triggered-PRM1,2; IS-PRM), corresponding to various scale format implementations on different quadrupole-Orbitrap instrument generations (Thermo Scientific™ Q Exactive™ HF MS and new Thermo Scientific™ Q Exactive™ HF-X MS), were evaluated, based on targeted proteomics experiments applied to signaling pathways.
Results: Recent technology developments, offered by the new Q Exactive HF-X mass spectrometer and the most advanced acquisition methods, provided substantial improvement in the scale and data quality of targeted quantification experiments. They allowed significant increase in depth and breadth of coverage in signaling pathway monitoring.
1 Thermo Fisher Scientific, France
2 Thermo Fisher Scientific, Bremen, DE
3 Carnegie Institution for Science, Stanford, CA
4 Thermo Fisher Scientific, Rockford, IL
5 Thermo Fisher Scientific, San Jose, CA