on 12-19-201812:39 AM - edited on 10-15-202108:22 AM by Closed Account
Tatjana D. Talamantes, Ioanna Ntai, Shannon Eluik, Amanda L. Souza
Purpose: To gain insight into the development of a universal LC-MS/MS method optimal for untargeted metabolomics discovery experiments.
Methods: Human Plasma (NIST SRM1950), E coli cell extract (Cambridge Isotopes), and Green Tea Extract (NIST SRM3255) were extracted and then analyzed with a Thermo Scientific™ Orbitrap Fusion™ Tribrid™ mass spectrometer. The data was processed using Thermo Scientific™ Compound Discoverer™ 3.0 software for metabolome coverage.
Results: A significant progress towards a universal untargeted metabolomics method has been accomplished. Here we present an insight into optimal conditions for maximal number of identifications in varying sample types.
Thermo Fisher Scientific, 355 River Oaks Pkwy, San Jose, CA, USA, 95134