Seema Sharma, Stephane Houel, Christopher Mullen, Chad Weisbrod, Romain Huguet, John Syka, Dave Horn, Jonathan Josephs, Jae Schwartz, Vlad Zabrouskov,
International Hupo 2016
Mass analysis and top-down characterization of a monoclonal antibody was conducted on a modified Orbitrap Fusion Lumos mass spectrometer. The mass spectrometer was fitted with a 213 nm wave-length Nd:YAG diode pumped solid state laser to allow for UVPD fragmentation in the linear trap. Multiple fragmentation modes were investigated and instrument parameters that influence the kinetics and extent of fragmentation were monitored with a goal to optimize sequence coverage.
Thermo Fisher Scientific, San Jose, CA
United States
