Perkel JM
Science 11 September 2015: Vol. 349 no. 6253 pp. 1243-1245
Between alternative transcription start sites, alternative splicing, and posttranslational modifications, a given gene may produce dozens of protein variants, each with a different biological activity. Teasing apart those structure-function relationships requires mapping specific variants to their associated biological functions, and the tool of the trade for doing so is mass spectrometry. But not just any mass spec
will do. Researchers need a holistic view of protein structure, data that is lost with the popular “bottom-up” proteomics strategy. Powered by today’s ultrahigh-resolution, high mass accuracy mass specs, protein biochemists are increasingly turning bottom-up upside-down. Their new alternative: top down proteomics.
https://www.sciencemag.org/content/349/6253/1243.summary
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