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The saliva proteome of the bloodfeeding insect Triatoma infestans is rich in platelet-aggregation inhibitors

Reputable Mentor II
Reputable Mentor II
Charneau S, Junqueira M, Costa CM, Pires DL, Fernandes ES, Bussacos AC, Sousa MV, Ricart CAO, Shevchenko A, Teixeira ARL.
Intl. J. Mass Spectrom. 2007, 268, 265-276
The saliva of the bloodsucking bug Triatoma infestans vector of Chagas disease contains an anti-hemostatic molecular cocktail that prevents coagulation, vasoconstriction and platelet aggregation in a vertebrate prey. In order to characterize T. infestans saliva proteome, we separated the secreted saliva by two-dimensional gel electrophoresis (2-DE). More than 200 salivary proteins were detected on the 2-DE map, mainly in the alkaline region. By nanoLC–MS/MS analysis using a LTQ–Orbitrap equipment followed by a combination of conventional and sequence-similarity searches, we identified 58 main protein spots. Most of such proteins possess potential blood-feeding associated functions, particularly anti-platelet aggregation proteins belonging to lipocalin and apyrase families. The saliva protein composition indicates a highly specific molecular mechanism of early response to platelet aggregation. This first proteome analysis of the T. infestans secreted saliva provides a basis for a better understanding of this fluid protein composition highly directed to counterpart hemostasis of the prey, thus promoting the bug's blood-feeding.
Laboratory of Biochemistry and Protein Chemistry, Department of Cell Biology, University of Brasília, Brasilia 70910-900-DF, Brazil
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