1Xiaoyue Jiang, 2Sergei Snovida, 1David Horn, 3Vic Spicer, 3Oleg Krokhin, 1Rosa Viner, 1Andreas Huhmer
ASMS 2017 Poster
Purpose: To develop a high accuracy, sensitivity, robustness and speed biomarker discovery and verification workflow based on a Tandem Mass Tag™ (TMT™) quantitation approach and transfer of retention time information into label-free quantitation using peptide retention prediction.
Methods: The depleted plasma from three normal and three diabetic patients was digested and analyzed in parallel using TMT-based and label-free methods. TMT-labeled peptides were fractionated and analyzed on a Thermo Scientific™ Orbitrap Fusion™ mass spectrometer (MS). Analysis of non-labeled peptide mixtures was performed using 1D LC-MS on a Thermo Scientific™ Q Exactive™ HF-X hybrid quadrupole-Orbitrap™ mass spectrometer.
Results: 48 potential biomarker proteins were found in diabetic patients using a TMT-based discovery workflow and validated by targeted label free peptide quantification.
1 Thermo Fisher Scientific, San Jose, CA
2 Thermo Fisher Scientific, Rockford, IL
3 University of Manitoba, Winnipeg, Canada
United States
