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The power of quantitative multiplexing-combining TMT discovery and targeted label free workflows for biomarker analysis

Reputable Mentor II
Reputable Mentor II
1Xiaoyue Jiang, 2Sergei Snovida, 1David Horn, 3Vic Spicer, 3Oleg Krokhin, 1Rosa Viner, 1Andreas Huhmer
ASMS 2017 Poster
Purpose: To develop a high accuracy, sensitivity, robustness and speed biomarker discovery and verification workflow based on a Tandem Mass Tag™ (TMT™) quantitation approach and transfer of retention time information into label-free quantitation using peptide retention prediction. Methods: The depleted plasma from three normal and three diabetic patients was digested and analyzed in parallel using TMT-based and label-free methods. TMT-labeled peptides were fractionated and analyzed on a Thermo Scientific™ Orbitrap Fusion™ mass spectrometer (MS). Analysis of non-labeled peptide mixtures was performed using 1D LC-MS on a Thermo Scientific™ Q Exactive™ HF-X hybrid quadrupole-Orbitrap™ mass spectrometer. Results: 48 potential biomarker proteins were found in diabetic patients using a TMT-based discovery workflow and validated by targeted label free peptide quantification.

1 Thermo Fisher Scientific, San Jose, CA 2 Thermo Fisher Scientific, Rockford, IL 3 University of Manitoba, Winnipeg, Canada
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Last update:
‎11-09-2021 01:41 AM
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