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The future key role of LC-high-resolution-MS analyses in clinical laboratories: a focus on quantification.

Reputable Mentor II
Reputable Mentor II
Rochat B, Kottelat E, McMullen J.
Bioanalysis. 2012 Dec;4(24):2939-58.
For the last decade, high-resolution (HR)-MS has been associated with qualitative analyses while triple quadrupole MS has been associated with routine quantitative analyses. However, a shift of this paradigm is taking place: quantitative and qualitative analyses will be increasingly performed by HR-MS, and it will become the common 'language' for most mass spectrometrists. Most analyses will be performed by full-scan acquisitions recording 'all' ions entering the HR-MS with subsequent construction of narrow-width extracted-ion chromatograms. Ions will be available for absolute quantification, profiling and data mining. In parallel to quantification, metabotyping will be the next step in clinical LC-MS analyses because it should help in personalized medicine. This article is aimed to help analytical chemists who perform targeted quantitative acquisitions with triple quadrupole MS make the transition to quantitative and qualitative analyses using HR-MS. Guidelines for the acceptance criteria of mass accuracy and for the determination of mass extraction windows in quantitative analyses are proposed.
Quantitative MS Facility, University Hospital of Lausanne, CHUV, 1011 Lausanne, Switzerland.
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‎10-15-2021 06:11 AM
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