A new LC system was specifically designed to reduce instrument maintenance, down time, and operating costs for high-throughput, LC/MS/MS applications which require sample cleanup prior to HPLC analysis. The Prelude SPLC System utilizes syringe pumps designed to deliver the volume of mobile phase required for each sample analysis with a single push of the piston. This pump design greatly reduces the wear and tear on pump seals and check valves, because the pistons in dual piston reciprocating pumps can move several hundred if not thousands of times per sample run.