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The Oxidized Proteome of Peripheral Blood Mononuclear Cells, A Valuable Repository for Clinical Proteomics

Reputable Mentor II
Reputable Mentor II
Xiaolei Xie1, Xiaoyue Jiang1, Monica Carrera2, Daniel Lopez-Ferrer1, Andreas FR Huhmer1

PBMCs and its specific cell subsets have allowed for a very broad collection of applications such as in vitro cell-based assays to study the immune response to a given stimuli, or to monitor in vivo or ex vivo changes before and after a drug treatment. More importantly, they are an easily accessible cellular part of the blood, and they are the only component of the blood that could have a gene expression activity. Access to complete atlas of the gene expression and posttranslational modifications in PBMCs will permit more sophisticated studies such us the selection of potential biomarkers that could be used for many purposes ranging from diagnostic, prognosis or even help selecting the appropriate therapy for a patient. This study compiles the most extensive proteome map of PBMCs. We have demonstrated that the combination of the results yielded the identification of over 8000 proteins. In addition, over 5000 proteins were accurately quantified and over 7000 oxidation events were identified. Remarkably, our data also suggest that H2O2 might play a role modulating signaling pathways by reacting with specific protein targets.Overall, this study not only adds significant value in the mechanistic understanding of redox signaling, but it also creates a valuable protein repository that could lead to the development of new therapeutic strategies.

1. Thermo Fisher Scientific, San Jose, USA 2. Spanish National Research Council (CSIC), Vigo, Spain
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Last update:
‎10-15-2021 11:34 AM
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