Targeted Quantitation of Insulin and Its Therapeutic Analogs for Research

[Orbitrap_SciLib]

Frahm J, Niederkofler EE, Peterman S, Schroeder T, Leber A, Antwi K, Tubbs KA, Krastins B, Prakash A, Lopez M.
ASMS 2014 Poster
Purpose: To perform simultaneous qualitative and quantitative analyses of endogenous insulin and/or therapeutic analogs at biological levels for research. Methods: We used a pan-anti insulin antibody in Thermo Scientific™ Mass Spectrometric Immunoassay (MSIA) D.A.R.T.’STM pipette tips for highly-selective affinity purification of all insulin analogs. Analogs were detected, verified, and quantified using high-resolution, accurate-mass (HRAM) MS and MS/MS data from a Thermo Scientific™ Q Exactive™ mass spectrometer. Results: We achieved a lower-limit-of-detection (LLOD) of 15 pM in plasma for all variants used with linear regressions of 0.99 or better. Further, we demonstrate interand intra-day CV’s of < 3% and spike and recovery resulted in recoveries of 96–100%.


Thermo Fisher Scientific