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Orbitrap_SciLib
Reputable Mentor II
Reputable Mentor II
Frahm J, Niederkofler EE, Peterman S, Schroeder T, Leber A, Antwi K, Tubbs KA, Krastins B, Prakash A, Lopez M.
ASMS 2014 Poster
Purpose: To perform simultaneous qualitative and quantitative analyses of endogenous insulin and/or therapeutic analogs at biological levels for research. Methods: We used a pan-anti insulin antibody in Thermo Scientific™ Mass Spectrometric Immunoassay (MSIA) D.A.R.T.’STM pipette tips for highly-selective affinity purification of all insulin analogs. Analogs were detected, verified, and quantified using high-resolution, accurate-mass (HRAM) MS and MS/MS data from a Thermo Scientific™ Q Exactive™ mass spectrometer. Results: We achieved a lower-limit-of-detection (LLOD) of 15 pM in plasma for all variants used with linear regressions of 0.99 or better. Further, we demonstrate interand intra-day CV’s of < 3% and spike and recovery resulted in recoveries of 96–100%.


Thermo Fisher Scientific
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