TMT Workflow on the Q Exactive Series - Instrument Parameter Optimization and Data Analysis in Proteome Discoverer 2.1 Software

[Orbitrap_SciLib]

Xiaoyue Jiang1, Tabiwang Arrey2, Eugen Damoc2, Michaela Scigelova2, David Horn1, Rosa Viner1, and Andreas F.R. Huhmer1

To develop an optimal acquisition method for relative quantification of proteins using Thermo Scientific™ TMTsixplex™ and TMT10plex™ workflows on the Thermo Scientific™ Q Exactive™ platforms. The topics of sample preparation, peptide separation and mass spectrometric analysis, and data processing using Thermo Scientific™ Proteome Discoverer™ software version 2.1 are covered in detail.


1. Thermo Fisher Scientific, San Jose, CA, USA 2. Thermo Fisher Scientific, Bremen, Germany