on 11-11-202001:40 PM - edited on 10-15-202111:45 AM by AnalyteGuru
Penny Jensen1, Bhavin Patel1, Aaron S. Gajadhar2, Sebastien Gallien3,4, Andreas Huhmer2, Daniel Lopez-Ferrer2, Kay Opperman1, John C. Rogers International Hupo 2020 The RAS/MAPK and AKT/mTOR pathways represent key mechanisms for cells to regulate cell survival, proliferation, and motility. The cross-talk between two pathways plays a central role in tumor progression and anti-cancer drug resistance. The quantitation of pathway protein expression and modifications are critical for characterization of disease, monitoring cancer progression and determining treatment response. A major limitation in the quantitation of pathway proteins is the lack of rigorously validated methods/reagents and a reliance on semi-quantitative results from Western blotting. We have utilized a novel SureQuant internal standard (IS)-triggered method applying a pool of reference internal standards to quantitate abundance of pathway proteins in a single MS run.
1Thermo Fisher Scientific, Rockford, IL
2Thermo Fisher Scientific, San Jose, CA
3Thermo Fisher Scientific, PMSC, Cambridge, MA
4Thermo Fisher Scientific, Paris, France.