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claudia-bouman
Team TFS
Team TFS
1) Department of Physical and Analytical Chemistry, University of Oviedo, Julian Claveria R, 33006 Oviedo, Spain, 2) Department of Biochemistry and Molecular Biology, University of Oviedo, Editicio Santiago Gascon, Campus de "El Cristo", 33006 Oviedo, Spain, 3) Innovative Solutions in Chemistry. Edificio Cientifico-Tecnológico, Campus de "El Cristo", 33006 Oviedo, Spain
Analytical Bioanalytical Chemistry (2013), 405(9), pp2889-99, doi: 10.1007/s00216-012-6420-x
Sulphur tracer experiments in laboratory animals using 34S-labelled yeast
Martínez-Sierra JG (1), Moreno Sanz F (2), Herrero Espílez P (2), Marchante Gayón JM (1), Rodríguez Fernández J (3), García Alonso JI (1)
We have evaluated the use of (34)S-labelled yeast to perform sulphur metabolic tracer experiments in laboratory animals. The proof of principle work included the selection of the culture conditions for the preparation of sulphur labelled yeast, the study of the suitability of this labelled yeast as sulphur source for tracer studies using in vitro gastrointestinal digestion and the administration of the (34)S-labelled yeast to laboratory animals to follow the fate and distribution of (34)S in the organism. For in vitro gastrointestinal digestion, the combination of sodium dodecyl sulphate-polyacrylamide gel electrophoresis and high-performance liquid chromatography and inductively coupled plasma mass spectrometry (HPLC-ICP-MS) showed that labelled methionine, cysteine and other low molecular weight sulphur-containing biomolecules were the major components in the digested extracts of the labelled yeast.
  • MC-ICPMS
  • Neptune Plus
  • Suphur Isotopes
  • HPLC
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