Zhang T, Hao Z, Horn D, Chen J, Zabrouskov V, Josephs J.
ASMS 2014 Poster
Purpose: Structural characterization of intact mAb method coupling capillary LC with new generation Orbitrap mass spectrometer.
Methods: An intact monoclonal antibody (mAb) molecule mass was measured by
Thermo Scientific™ Orbitrap™ Fusion™ MS. Mass spectra of the intact protein were analyzed by Thermo Scientific™ Protein Deconvolution™ software. Both Thermo Scientific™ Orbitrap Elite™ MS and Orbitrap Fusion MS systems were used for mAb top-down analysis. ETD and HCD MS/MS data were analyzed by Thermo Scientific™ ProSightPC™ 3.0 software.
Results: mAb was accurately measured with low ppm mass error and multiple
glycoforms were accurately identified. About 70% backbone sequence coverage for light chain and 20% for heavy chain was achieved using top-down analysis.
Thermo Fisher Scientific
United States
