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Orbitrap_SciLib
Reputable Mentor II
Reputable Mentor II
Zhang T, Hao Z, Horn D, Chen J, Zabrouskov V, Josephs J.
ASMS 2014 Poster
Purpose: Structural characterization of intact mAb method coupling capillary LC with new generation Orbitrap mass spectrometer. Methods: An intact monoclonal antibody (mAb) molecule mass was measured by Thermo Scientific™ Orbitrap™ Fusion™ MS. Mass spectra of the intact protein were analyzed by Thermo Scientific™ Protein Deconvolution™ software. Both Thermo Scientific™ Orbitrap Elite™ MS and Orbitrap Fusion MS systems were used for mAb top-down analysis. ETD and HCD MS/MS data were analyzed by Thermo Scientific™ ProSightPC™ 3.0 software. Results: mAb was accurately measured with low ppm mass error and multiple glycoforms were accurately identified. About 70% backbone sequence coverage for light chain and 20% for heavy chain was achieved using top-down analysis.


Thermo Fisher Scientific
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‎10-15-2021 12:05 PM
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