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Structure Characterization of Intact Monoclonal Antibody Using an Orbitrap Tribrid Mass Spectrometer

Reputable Mentor II
Reputable Mentor II
Zhang T, Hao Z, Horn D, Chen J, Zabrouskov V, Josephs J.
ASMS 2014 Poster
Purpose: Structural characterization of intact mAb method coupling capillary LC with new generation Orbitrap mass spectrometer. Methods: An intact monoclonal antibody (mAb) molecule mass was measured by Thermo Scientific™ Orbitrap™ Fusion™ MS. Mass spectra of the intact protein were analyzed by Thermo Scientific™ Protein Deconvolution™ software. Both Thermo Scientific™ Orbitrap Elite™ MS and Orbitrap Fusion MS systems were used for mAb top-down analysis. ETD and HCD MS/MS data were analyzed by Thermo Scientific™ ProSightPC™ 3.0 software. Results: mAb was accurately measured with low ppm mass error and multiple glycoforms were accurately identified. About 70% backbone sequence coverage for light chain and 20% for heavy chain was achieved using top-down analysis.

Thermo Fisher Scientific
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Last update:
‎10-15-2021 12:05 PM
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