on 05-01-201210:54 AM - edited on 10-15-202112:07 PM by AnalyteGuru
Tsybin Y, Damoc E, Fornelli L, Miladinovic S, Nolting D, Zeller M, Grouzmann E, Makarov A. ASMS 2011 Poster • ETD on Orbitrap FTMS produced 162 identified unique backbone cleavages sites on ~150 kDa intact protein (IgG) on the LC timescale.
• Sequence coverage obtained by ETD is generally higher than the one obtained by slow-heating activation methods and, importantly, provides valuable information on the variable domains.
• ETD fragmentation patterns of IgGs derived from different organisms (i. e., with significant differences in sequence-structure) show relevant differences in the number and abundances of product ions, especially in the 200-1200 m/z region.
• Further improvement in S/N is required for identification of PTMs and increased sequence coverage.