Structural Analysis of Labeled N-Glycans from Proteins by LC-MS/MS Separated Using a Novel Mixed-Mode Stationary Phase

[steve-day]

This application note describes the liquid chromatography-mass spectrometry (LC/MS) analysis of fluorescently labeled N-glycans released from proteins. The chromatographic separation is carried out with a novel Thermo Scientific™ GlycanPac™ AXH-1 (1.9 µm, 2.1 × 150 mm) column for high-resolution and high-throughput analysis of glycans.