on 06-12-201804:13 AM - edited on 10-15-202111:37 AM by AnalyteGuru
David A Peake1, Reiko Kiyonami1, Daniel Gachotte2, Gavin E Reid3, Yasuto Yokoi4, and Andreas Hühmer1 ASMS 2018 Thermo Scientific™ LipidSearch™ software version 4.2 for untargeted lipidomics with LC dd-MSn analysis provides enhanced lipid characterization by combining information obtained in HCD and CID MS2 and MS3 product ion spectra. This lipid workflow is demonstrated on a new Tribrid™ Orbitrap™ mass spectrometer designed for characterization of small molecules such as metabolites and lipids. Total lipid extracts from western corn rootworm larvae were analyzed giving high confidence in lipid annotations and good reproducibility for relative and estimated quantitation of almost one thousand lipid species. Phosphatidylcholine and triglyceride lipids were automatically targeted by specific product ion or neutral losses, which enabled further CID MS2/MS3 characterization during the same dd-MS2 acquisition cycle. Functional lipids such as PI isomers and sterol esters are readily annotated and quantified at low levels. Different phenotypes are observed between two isomeric fatty acyl combinations: PI 18:1_18:3 and PI 18:2/18:2.
1 Thermo Fisher Scientific, San Jose, CA, United States
2 CORTEVA Agriscience, Indianapolis, IN, United States
3 The University of Melbourne, Parkville, Victoria, Australia
4 Mitsui Knowledge Industry, Atago, Tokyo, Minato-ku, Japan