on 05-01-201210:48 AM - edited on 10-15-202107:18 AM by AnalyteGuru
Self RL, Wu WH, Marks HS. J Agric Food Chem. 2011 Jun 8;59(11):5906-13. A method for the extraction of agmatine, cadaverine, histamine, phenyethylamine, putrescine, tryptamine, tyramine, and urocanic acid from canned tuna and frozen tuna loin matrices by matrix solid-phase dispersion, followed by separation and quantification of these compounds by ultrahigh-performance hydrophilic interaction chromatography (UHPLC-HILIC) with orbitrap mass spectrometric detection, is described. Tuna samples are dispersed in a CN-silica sorbent and eluted with a mixture of aqueous ammonium formate buffer and acetonitrile. Separation and detection are carried out on an Agilent 1200 high-performance liquid chromatograph coupled to a Thermo Exactive orbitrap mass spectrometer, and metformin is used as the internal standard. Spike recoveries are determined across a range of 20–100 ppm for each compound, and the method is validated with respect to linearity, reproducibility, accuracy, and limits of quantitation and detection. The method is demonstrated to be suitable for use in quantifying these target compounds in the studied matrices.
http://pubs.acs.org/doi/abs/10.1021/jf200455r Applied Technology Center, Pacific Regional Laboratory Northwest, Office of Regulatory Affairs, U.S. Food and Drug Administration, 22201 23rd Drive S.E., Bothell, Washington 98021, USA.